Canine Serum

The purpose of this study was to develop and validate analytical enzyme linked immunosorbent assay (ELISA) for the measurement of canine gastric lipase immunoreactivity (CGLI). Sandwich ELISA was developed using the dog gastric lipase (CGL) was purified from the dog's stomach and polyclonal antibodies directed against the CGL, raised in rabbits and purified by affinity chromatography.  This test is validated  by  Mouse Serum Albumin determination of sensitivity, working range, linearity, accuracy, precision, reproducibility, and the upper limit of the control range to determine CGLI 97.5 percentile serum concentrations in 74 healthy canines. Sensitivity and outreach work in the serum of 200 ng / L and 200-39160 ng / L, respectively. Observed ratio is expected to dilutional parallelism for 3 and 3 dilution of serum samples ranged from 86.1% to 244.2% (mean +/- standard deviation [s]; 125.4% +/- 48.2%). Observed ratios for spiking recovery expected for 3 serum samples and ...

Validation for canine serum of 2 commercially available fluoroimmunoassays time-resolved (TR-FIAS) is designed for analysis of cortisol and free thyroxine (FT4) in human serum do. Included is the study of interference by hemolysis, lipemia, and bilirubinemia. By dissociation enhancement lanthanide fluoroimmunoassay kit, intra-assay coefficient of variation (CV) ranged from 6.4% to 8.7% for cortisol and from 5.3% to 9.8% for the FT4; Interassay CVs ranged from 5.8% to 10.8% and from 3.9% to 14.1%, respectively.  Accuracy was evaluated by  Mouse Plasma comparing cortisol and FT4 results obtained with TR-FIA and obtained by enzyme-linked immunosorbent assay validated (ELISA) and equilibrium dialysis (ED) assay, respectively. The regression equation obtained y = 0.57x + 1:18 (r2 = 0.90) for cortisol and y = 0.87x + 0.82 (r2 = 0.93) for FT4.  The limit of detection for cortisol and FT4 was 4.84 nmol / L and 2.68 pmol / L, respectively. Results adrenocorticotropin-stimulation a...

The purpose of this study was to determine the effect of hemolysis, lipemia, bilirubinemia, and anticoagulants may be the most commonly used test for C-reactive protein and serum amyloid A, and determination of ceruloplasmin in dogs. Solution hemoglobin, lipids, and serum bilirubin added to aliquots. In addition, serum and plasma samples with different anticoagulants (heparin, EDTA, and citrate) were obtained from healthy dogs.  Hemolysis, lipemia, and hyperbilirubinemia significantly  Human Serums interfere with protein and ceruloplasmin results of C-reactive, but not with people to test serum amyloid A. The use of anticoagulants resulted in significant changes in the results of the test were tested. However, the magnitude of the difference caused by interfering substances not seem to have an important impact on the clinical interpretation of the test. The effects of dog serum were collected from the dogs at different stages of the estrous cycle in vitro canine oocyte nuclea...

BACKGROUND Monitoring systemic inflammatory activity during steroid therapy of canine immune polyarthritis (IMPA) difficult and mainly rely on clinical signs. METHOD Canine serum C-reactive protein (CRP) were measured serially and blinded during follow-up 27 weeks of Anaplasma case phagocytophilia induced type II immune-mediated polyarthritis. CONCLUSION WBC was, as expected , does  Human Serum not reflect the observed inflammatory activity during steroid treatment with a clinically useful manner, whereas, suggested CRP contains valuable marker inflammatory activity during steroid treatment in this case. elevated serum urea: creatinine ratio in canine babesiosis in South Africa is not the origin of the kidney. Serum pigmented, usually due to free hemoglobin and / or bilirubin, is a common finding in dogs with babesiosis, resulting in disruption of all biochemical tests that rely on photochemistry. This is especially true of urea and creatinine determinations, complicate the diagno...

he present study evaluated the effects of erythrocytes infected culture supernatant, fractionation of culture supernatant and serum of dogs infected with Babesia gibsoni (B. gibsoni) on in vitro maturation of reticulocytes dog. SDS-PAGE shows that a significantly wider band produced by both culture supernatant of infected erythrocytes and serum from chronically infected with B. gibsoni dog.  Culture supernatant of erythrocytes infected  Hamster Serum with B. gibsoni greatly suppressed the maturation of reticulocytes. previous studies have shown that chronically infected serum has an inhibitory effect on the maturation of reticulocytes and dogs pyrimidine 5'-nucleotidase special subclass I and purine 5'-nucleotidase activity. In addition, serum-free culture supernatant of infected erythrocytes have an inhibitory effect on the morphological maturation of reticulocytes.  These results indicate that the infected serum and erythrocyte culture supernatant may accumulate excess...

Neutrophil gelatinase-associated lipocalin (NGAL) is a glycoprotein is involved in inflammation acts as an acute phase protein and chemokines as well as a regulator of iron homeostasis. NGAL has proved to be upregulated in experimental autoimmune encephalomyelitis (EAE) in mice.  Increasing concentrations of NGAL in  Goat Serum Albumin the cerebrospinal fluid (CSF) and the expression in the central nervous system (CNS) have been described in human neuroinflammatory diseases such as multiple sclerosis and lupus, neuropsychiatric and bacterial meningitis. We aimed to investigate the involvement of NGAL in spontaneous nerve inflammation dog as an animal model of the potential immune-mediated neurological disorders.  A commercially available enzyme-linked immunosorbent assay (ELISA) for the detection of NGAL dog CSF validated for use in dogs. Concentration in CSF and serum of patients suffering from steroid-responsive dog meningitis- arteriitis (SRMA), Meningoencephalitis of...