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Development and analytical validation of an enzyme linked immunosorbent assay for the measurement of canine gastric lipase immunoreactivity in serum.

The purpose of this study was to develop and validate analytical enzyme linked immunosorbent assay (ELISA) for the measurement of canine gastric lipase immunoreactivity (CGLI). Sandwich ELISA was developed using the dog gastric lipase (CGL) was purified from the dog's stomach and polyclonal antibodies directed against the CGL, raised in rabbits and purified by affinity chromatography. 

This test is validated by Mouse Serum Albumin determination of sensitivity, working range, linearity, accuracy, precision, reproducibility, and the upper limit of the control range to determine CGLI 97.5 percentile serum concentrations in 74 healthy canines. Sensitivity and outreach work in the serum of 200 ng / L and 200-39160 ng / L, respectively. Observed ratio is expected to dilutional parallelism for 3 and 3 dilution of serum samples ranged from 86.1% to 244.2% (mean +/- standard deviation [s]; 125.4% +/- 48.2%). Observed ratios for spiking recovery expected for 3 serum samples and 6 spiking concentrations ranged from 66.4% to 152.5% (mean +/- s; 104.5% +/- 22.9%). 

Intra-assay and interassay variability for 3 different serum samples was 25.5%, 9.4%, and 13.4% and 26.0%, 17.2% and 14.4%, respectively. The upper limit of the control range for serum CGLI is 662 ng / L. We conclude that ELISA for CGLI described here are very sensitive and show a variety of work. However, the characteristics of validation for this test is suboptimal and below the value of approximately 2,000 ng / L assay is semi-quantitative in nat
Development and analytical validation of an enzyme linked immunosorbent assay for the measurement of canine gastric lipase immunoreactivity in serum.
ure. Despite the limitations, if this test is useful for the diagnosis of canine gastric disorders remains to be determined.


Serum antibody titer for canine parvovirus, adenovirus and distemper virus in dogs in the UK who have not been vaccinated for at least three years.


titer antibodies to canine distemper (CDV), canine parvovirus (CPV) and canine adenovirus (CAV) was measured in 144 adult dogs who have not been vaccinated for between three and 15 years. CPV protective antibodies are present in 95 percent of the population, for CDV at 71.5 percent and for CAV at 82 percent. 

The prevalence of protective titer does https://gentaur.cz/ not decrease with increasing the time interval from the last vaccination for one of the three diseases studied. Booster vaccination titer increases CAV dog. For comparison purposes, 199 puppies were sampled during their first and second vaccinations. 

In the case of CPV and CAV significantly higher proportion of adult dogs than puppies are protected as soon as they are vaccinated. Natural CPV increase was allegedly because the dogs had significantly higher titers of three years after primary vaccination them two weeks later and three unvaccinated dogs had acquired protective antibody levels uneventfully. There is no evidence of natural exposure to CDV.
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