Skip to main content

Measurement of Neutrophil Gelatinase-Associated Lipocalin Concentration in Canine Cerebrospinal Fluid and Serum and Its Involvement in Neuroinflammation.

Neutrophil gelatinase-associated lipocalin (NGAL) is a glycoprotein is involved in inflammation acts as an acute phase protein and chemokines as well as a regulator of iron homeostasis. NGAL has proved to be upregulated in experimental autoimmune encephalomyelitis (EAE) in mice.

 Increasing concentrations of NGAL in Goat Serum Albumin the cerebrospinal fluid (CSF) and the expression in the central nervous system (CNS) have been described in human neuroinflammatory diseases such as multiple sclerosis and lupus, neuropsychiatric and bacterial meningitis. We aimed to investigate the involvement of NGAL in spontaneous nerve inflammation dog as an animal model of the potential immune-mediated neurological disorders.

 A commercially available enzyme-linked immunosorbent assay (ELISA) for the detection of NGAL dog CSF validated for use in dogs. Concentration in CSF and serum of patients suffering from steroid-responsive dog meningitis- arteriitis (SRMA), Meningoencephalitis of unknown origin (Muo), different non-inflammatory and CNS disease compared to control dogs. 

The relationship between the concentration in CSF and serum NGAL and inflammatory parameters in CSF and blood (IgA concentration, the total number of nucleated cells (TNCC), protein content) as well as the association with erythrocytes in CSF, duration of disease, plasma creatinine and urine leukocytes evaluated. 
Measurement of Neutrophil Gelatinase-Associated Lipocalin Concentration in Canine Cerebrospinal Fluid and Serum and Its Involvement in Neuroinflammation.

In dogs with SRMA and Muo, CSF concentrations of NGAL were significantly higher than in dogs with idiopathic epilepsy, press myelopathy, intracranial neoplasia and SRMA in remission (p <0.0001). Patients with acute SRMA had significantly higher levels of NGAL in the CSF of neurologically normal controls (p <0.0001). serum concentrations of NGAL were significantly higher in dogs with SRMA compared to patients with myelopathy and intracranial neoplasia (p <0.0001). 

NGAL levels in CSF were positive correlated Hamster Plasma with the concentration of IgA (rSpear = 0.60116, p <0.0001), TNCC (rSpear = 0.65746, p <0.0001) and protein levels (rSpear = 0.73353, p <0.0001) in the CSF. It can be measured in the CSF of healthy dogs and sick. higher concentrations in patients dogs with SRMA and positive relationship with TNCC in CSF suggests involvement in pro-inflammatory pathways and chemotaxis in SRMA. high serum NGAL levels in serum of patients SRMA in various stages of the disease may reflect systemic character of the disease.

Comments

Popular posts from this blog

Suppressive effect of culture supernatant of erythrocytes and serum from dogs infected with Babesia gibsoni on the morphological maturation of canine reticulocytes in vitro.

he present study evaluated the effects of erythrocytes infected culture supernatant, fractionation of culture supernatant and serum of dogs infected with Babesia gibsoni (B. gibsoni) on in vitro maturation of reticulocytes dog. SDS-PAGE shows that a significantly wider band produced by both culture supernatant of infected erythrocytes and serum from chronically infected with B. gibsoni dog.  Culture supernatant of erythrocytes infected  Hamster Serum with B. gibsoni greatly suppressed the maturation of reticulocytes. previous studies have shown that chronically infected serum has an inhibitory effect on the maturation of reticulocytes and dogs pyrimidine 5'-nucleotidase special subclass I and purine 5'-nucleotidase activity. In addition, serum-free culture supernatant of infected erythrocytes have an inhibitory effect on the morphological maturation of reticulocytes.  These results indicate that the infected serum and erythrocyte culture supernatant may accumulate excess...

Cortisol and free thyroxine determination by time-resolved fluorometry in canine serum.

Validation for canine serum of 2 commercially available fluoroimmunoassays time-resolved (TR-FIAS) is designed for analysis of cortisol and free thyroxine (FT4) in human serum do. Included is the study of interference by hemolysis, lipemia, and bilirubinemia. By dissociation enhancement lanthanide fluoroimmunoassay kit, intra-assay coefficient of variation (CV) ranged from 6.4% to 8.7% for cortisol and from 5.3% to 9.8% for the FT4; Interassay CVs ranged from 5.8% to 10.8% and from 3.9% to 14.1%, respectively.  Accuracy was evaluated by  Mouse Plasma comparing cortisol and FT4 results obtained with TR-FIA and obtained by enzyme-linked immunosorbent assay validated (ELISA) and equilibrium dialysis (ED) assay, respectively. The regression equation obtained y = 0.57x + 1:18 (r2 = 0.90) for cortisol and y = 0.87x + 0.82 (r2 = 0.93) for FT4.  The limit of detection for cortisol and FT4 was 4.84 nmol / L and 2.68 pmol / L, respectively. Results adrenocorticotropin-stimulation a...