Effects of hemolysis, lipemia, hyperbilirrubinemia, and anticoagulants in canine C-reactive protein, serum amyloid A, and ceruloplasmin assays.

The purpose of this study was to determine the effect of hemolysis, lipemia, bilirubinemia, and anticoagulants may be the most commonly used test for C-reactive protein and serum amyloid A, and determination of ceruloplasmin in dogs. Solution hemoglobin, lipids, and serum bilirubin added to aliquots. In addition, serum and plasma samples with different anticoagulants (heparin, EDTA, and citrate) were obtained from healthy dogs.

 Hemolysis, lipemia, and hyperbilirubinemia significantly Human Serums interfere with protein and ceruloplasmin results of C-reactive, but not with people to test serum amyloid A. The use of anticoagulants resulted in significant changes in the results of the test were tested. However, the magnitude of the difference caused by interfering substances not seem to have an important impact on the clinical interpretation of the test.

The effects of dog serum were collected from the dogs at different stages of the estrous cycle in vitro canine oocyte nuclear maturation.

canine oocytes ovulated at prophase of the first meiotic division and undergo maturation in the distal portion of the oviduct for at least 48-72 hours. Because of this difference from other domestic mammals, the efficiency of in vitro maturation (IVM) of canine oocytes is very low. This study was conducted to evaluate the effects of dog serum on IVM oocytes recovered from ovaries dogs in various countries of reproduction (follicular, luteal or anestrous stage). 

Oocytes were found chopped ovaries of prostitutes presented for ovariohysterectomy at various stages of the estrous cycle. Heat-inactivated serum dog ready with blood taken from dogs in anestrous stage, estrus or diestrous of the estrous cycle as determined by the concentration of progesterone and vaginal cytology. Oocytes were cultured for 72 hours in tissue culture medium (TCM) supplemented with 10% -199 anestrous dog, estrus or diestrous serum or fetal bovine serum (FBS) (Experiment 1), or supplemented with 0 (control), 5%, 10% or 20% serum dog estrus (experiment 2).

 In experiment 1, IVM oocytes were Monkey Serum Albumin collected in the follicular phase of the estrous cycle to metaphase II (MII) stage was higher (p <0.05) with serum estrus dogs (14.2%) compared to dogs anestrous (5.2%) or diestrous serum (6.3%), FBS (2.2%) or control (2.2%). In experiment 2, oocytes were collected in the follicular phase of the estrous cycle cultured in TCM-199 with 10% serum estrus dogs showing a higher maturity level to MII stage (13.5%, p <0.05) compared with those grown by 5% (1.3% MII) or 20% estrus dog serum (5.1% MII) or control (2.7% MII). In conclusion, our results suggest that the culture medium supplemented with 10% serum dog estrus increase IVM of canine oocytes follicular phase.